C-Fern life cycle
St Mary”s University Faculty of Science
C-Fern life cycle
Discipline: Biology
Type of service: Lab Report
Spacing: Double spacing
Paper format: MLA
Number of pages: 1 page
Number of sources: 2 sources
Paper details:
This is the second part of the lab report for a previous order, this part is the material and methods and I will provide the lab manual we’re using for the experiment and the instructions to be followed. It is required to include the microscope brand name so just keep the brand name blank and I will include it myself. There isn’t a specific number for resources. Here’s the material used: Petri dish, Spore spreader (sterile), volumetric pipette, culture dome, microscope, slides and coverslips, spores solution. 4ml distilled water, tooth pick. This is all what I can remember but you can fix it if I’m mistaken! and for the methods, I believe it is described in the manual below.
PLANT BIOLOGY
BIOL 2303 – Spring 2018
Preparing the Materials and Methods
This assignment is designed to help you get started on your lab report that is due at the end of the semester. You have already prepared and received feedback on your Introduction. You will now prepare the Materials and Methods for this report. The feedback that you receive on your method writing will help you to produce a well thought out and written final submission.
This section of a lab report should include a concise description of the materials, procedures, and equipment that you used. It should also include how the study was conducted, how the data were collected, and any statistical or graphical analyses that were completed.
The Materials and Methods outline what was done and how it was done. There should be enough detail so that someone else could repeat your experiment. The arrangement should be logical (i.e. keep related tasks and procedures to and write chronologically). This section is to be written in paragraph form and should not appear as bullet points or lists.
Keep in mind that you will need to include how the experiment is set up (i.e. how the plates are being stored, under what lighting conditions, the approximate temperature of the room, etc.)
Additionally, you should continue to update your working abstract to include information related to the materials and methods.
Be sure to include:
- Brand names of equipment when appropriate
- g. C-Fern® Investigations: Sex in a Dish, Carolina Biological Supply Company
- Concentrations and quantities of solutions
- g. 4 mL of sterile distilled water were added to the vial of dry Ceratopteris spores and agitated to ensure even suspension of spores in solution
- The organism(s) studied, their scientific name(s), numbers, size, sex, etc.
- g. Spores from Ceratopteris richardii were obtained from…
- g. On day 14, the incubating petri plate was removed from the humidity dome and examined under a stereoscope (brand name). Hermaphroditic and male thalli….
- Primary methods used
- Techniques, methods, and any appropriate figures
- Any formulas or calculations
- Statistical analyses
If you were following specific directions from a book, manual, or another research study, cite references those details. If any changes were made to those laid out in the manual that you may make reference to, indicate those differences in your report and briefly explain why the changes were made.
You SHOULD NOT include the rational for your work here; you will have time to discuss that in the appropriate section of the report.
Materials and Methods Formatting:
- Past tense, passive or active voice
- g. “we observed the spores…” or “the spores were observed”
- Paragraphs
- Times New Roman 12pt font
- Double space
- Indent at the beginning of paragraphs
ABSTRACT
Your abstract will appear here. You will not be completing the abstract in full for this portion of the assignment, but you will be completing an abstract for the final submission. The abstract is the part of the paper that tells the reader whether or not they are interested in looking at the rest of the paper. Therefore, the information you present in the abstract should show the reader the important and main findings of the work. The abstract summarizes your experiment and consists of 1 or 2 sentences for each of the major sections of the report (introduction, methods, results, and discussion). Although the abstract appears first in the report, you will prepare this section last.
INTRODUCTION
Your prepared introduction will appear here. When you submit your introduction, you should include the above abstract section with the main points from your introduction included. This will allow your TA the opportunity to provide feedback on your ability to distill the information in the introduction down to a few important statements. You should be including in-text referencing of your reference material. It should appear as such (Fisher 2015).
Only indent the second and subsequent paragraphs in the body of your paper.
MATERIALS AND METHODS
In this section, I have included a short excerpt from my graduate thesis work. I believe that is clearly illustrates the appropriate detail that is required in method writing.
BIOLOG® EcoPlatesTM (BioLog, Hayward, CA, USA) were employed to characterize and compare the phylloepiphytic communities associated with CG, FG, and PK leaves. The system is comprised of a pre-formatted 96 well microplate containing 3 replicates of 31 carbon substrates and a control well containing no carbon source (Figure 12). Each well was filled with 150 μL of undiluted leaf extract (prepared as described in section 3.2.4) from untreated control leaves. Measurement of substrate metabolism was based on colour development in each well resulting from the reduction of tetrazolium dye (Wang et al., 2007). Absorbance readings were taken on a BIOLOG® plate reader 76 (MicroStation ID Station, BioLog, Hayward, CA, USA) after 24 hours of incubation at 30°C. Diversity indices (DI = number of positive wells/total number of wells x 100) and average well colour development (AWCD = Ʃ (C-R) / n where C is colour production within each well, and R is the absorbance reading of the water-filled control well, and “n” is the number of substrates) were calculated for each leaf source. In order to establish similarities in carbon source utilization between epiphytic bacteria and E. coli 700728Na, pure cultures of the latter (ca. 1×105 CFU/100 μL) were placed into a separate EcoPlateTM and incubated in the same manner.
REFERENCES
Arnold RM, Slyker JA, Gupta TH. 1996. Germination of Cheaenorrhinum seeds in response to gibberellin treatments, J Plant Physiol. 148(6):677-683.
Baskin CC, Baskin JM. 1998. Seeds: ecology, biogeography, and evolution of dormancy and germination. New York: Academic Press.